1.    Treatment with 10% potassium hydroxide

2.     Positive stain with

a. Lactophenol cotton blue

b. Grocott silver stain

c.   Hematoxylin

d.    Eosin
 

3.    Negative stain with India ink

C.    Culture of fungi on

1. Sabouraud's agar (favors fungal growth because of low pH)

2. Mycosel agar (selective for pathogenic fungi because of chloramphenicol and cycloheximide in medium)

1.    Colonial morphology

2.    Cellular morphology
 

a.    Hyphal morphology
 
(1)    Aseptate or coenocytic

(2)    Septate

(a)    Regular connection

(b)    Clamp connection


b.    Spore morphology

(1) Conidiospore

(2) Sporangiospore

(3) Arthrospore

(4) Chlamydospore


c.    Yeast morphology

(1)    Size

(2)    Thickness of walls

(3)    Capsule presence/absence

1.    Biochemical tests

2.    Behavior in broth and serum (germ tube formation)

3.    Behavior on cornmeal agar (pseudohyphae formation)

Information provided by: http://www.kcom.edu